The microencapsulation of the flavonoid genistein with soluble chitosan allows efficient release in colon and attenuation of oxidative stress during experimental colitis.

VANDEN BRABER NL, NOVOTNY NUÑEZ I, BOHL L, PORPORATTO C, MONTENEGRO M, CORREA SG

Mar del Plata

Congreso; Reunion Anual Conjunta SAIC-SAI-SAFE.; 2016

SAIC-SAI-SAFE

Genistein (G) is a flavonoid with antioxidant, anti-inflammatory and anti-tumoral properties whose absorption occurs in the stomach and small intestine with significant loss of the bioactivity. The hypothesis of the present work was that upon microencapsulation, G could modulate the inflammatory process in the classic colitis model. Colitis was induced by administration of 3% (w / v) dextran sulfate sodium in drinking water for 5 days. G was microencapsulated (MCG) in water soluble chitosan (WSCh) by spray-drying. Female C57BL /6 mice were divided into 5 groups: Control, Colitis, Colitis+G, Colitis+MCG and Colitis+WSCh. G , MCG and WSCh were administered orally for 7 days starting at the beginning of colitis induction and animals were clinically evaluated during 10 days. The disease activity index (DAI) was calculated through weight loss, consistency and bleeding stool. Colon was isolated and processed for pro and anti-inflammatory cytokine analysis and superoxide dismutase (SOD), catalase and myeloperoxidase (MPO) activities. Statistical analyses were performed by ANOVA/Tukey. In MCG treated animals, the DAI was 70% lower than in Colitis group (p<0.01) while Colitis+G did not show significant improvements. Levels of IL-6, TNF-α and MCP-1 decreased with MCG administration (p<0.01) while a significant increment of IL-10 was found. Colitis+WSCh group showed no remarkable clinical recovery although a marked decrease in pro-inflammatory cytokines and oxidative stress was observed. SOD, catalase and MPO activities in Colitis+MCG group were comparable to Control. Together, the microencapsulation strategy allowed the delivery of the bioactive flavonoid to the colon with an effective attenuation of the inflammation as well as the recovery of the oxidative balance.