An anticatalase lentiviral vector reduced ethanol intake in developmentally-lead-exposed rats

MATTALLONI, MS; SALINAS C; QUINTANILLA ME; HERRERA-MARSCHITZ M; ISRAEL Y; CANCELA L; RIVERA-MEZA, M; VIRGOLINI MB

ALCOHOL AND ALCOHOLISM

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2015 vol. 50 p. 44 - 44

0735-0414

revious reports indicate that developmentally-lead (Pb)-exposed rats consumed more ethanol than theircontrol counterparts. Based on pharmacological and biochemical evidences, we attribute these differencesto catalase (CAT), the main enzyme involved in brain ethanol oxidation to acetaldehyde, a key componentin ethanol´s positive reinforcing effects. Thus, in the present study we sought to prevent brain CATexpression by the microinfusion of an antiCAT lentiviral vector in the ventral tegmental area (VTA) in orderto evaluate its impact on voluntary ethanol intake in control and Pb-exposed animals. To this end, apshRNA antiCAT vector was generated, packed in HEK 293T cells, and titrated by quantitative PCR.Thirty-five-day-old male Wistar rats perinatally exposed to 220 ppm Pb or vehicle with 2 h/day access towater or increasing concentrations of ethanol (2-10% v/v) were microinfused with the lentiviral vector atthe beginning (day 1) or