Protective effect of quercetin in gentamicin-induced oxidative stress in vitro and in vivo in blood cells. Effect on gentamicin antimicrobial activity

BUSTOS PS; DEZA-PONZIO R; PAEZ PAULINA; ALBESA I; CABRERA JL; VIRGOLINI MB; ORTEGA MG

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 48 p. 253 - 253

1382-6689

e have evaluated the effect of gentamicin and gentamicin plus quercetin on ROS production,endogenous antioxidant defenses (SOD and CAT) and lipid peroxidation in vitro on humanleukocytes and in vivo on whole rat blood. Gentamicin generated ROS production in humanleukocytes, produced a dual effect on both enzymes dosage-dependent and generated an increasein lipid peroxidation. Quercetin, in leukocytes stimulated by gentamicin, showed more inhibitorycapacity in ROS production than the reference inhibitor (vitamin C) in mononuclear cells and asimilar protective behavior at this inhibitor in polymorphonuclear cells. Quercetin, in bothcellular systems, tend to level SOD and CAT activities, reaching basal values and could preventlipidic peroxidation induced by gentamicin. The results in Wistar rats confirmed that therapeuticdoses of gentamicin can induce oxidative stress in whole blood and that the gentamicin treatmentplus quercetin can suppress ROS generation, collaborate with SOD and CAT