Brain ethanol-metabolizing enzymes are differentially expressed in lead-exposed animals after voluntary ethanol consumption: Pharmacological approaches.

MATTALLONI MS.; DEZA-PONZIO R; ALBRECHT PA; FERNANDEZ HUBEID, LE; CANCELA, L.M.; VIRGOLINI MB

NEUROTOXICOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019

0161-813X

evelopmentally-lead (Pb)-exposed rats showed an enhanced vulnerability to the stimulating and motivationaleffects of ethanol (EtOH). This is accompanied by differential activity of the brain EtOH-metabolizing enzymescatalase (CAT) and mitochondrial aldehyde dehydrogenase (ALDH2). Based on the theory that brain acetaldehydeaccumulation is associated with the reinforcing properties of EtOH, this study sought to determine brainCAT and ALDH2 expression in limbic areas of control and Pb-exposed animals after voluntary EtOH intake.Thirty-five-day-old rats perinatally exposed to 220ppm Pb were offered with water or increasing EtOH solutions(2?10% v/v) during 28 days until postnatal day (PND) 63. Once intake was stable, the animals were administered:1) saline (SAL; test days 21?24 or 21?28, as corresponds), or 2) a CAT inhibitor: 3-amine 1, 2, 4-triazole(AT; 250mg/kg intraperitoneally [i.p.], 5h before the last eight EtOH intake sessions -test days 21?24 and25?28), or 3) a CAT booster: 3-nitr