VIRGOLINI MIRIAM BEATRIZ
Congresos y reuniones científicas
Título:
Cannabinoid CB1 Receptors within Nucleus Accumbens Shell Are Not Involved in Stress-Induced Reinstatement in Extinguished Cocaine? Conditioned Animals
Autor/es:
GUZMAN AS; DE GIOVANNI L; AVALOS MP; BOLLATI FA; VIRGOLINI MB; CANCELA LM
Lugar:
MAR DEL PLATA
Reunión:
Congreso; XXX Annual Meeting and SAN-ISN Small Conference and Course; 2015
Institución organizadora:
SAN
Resumen:
Endocannabinoid system, primarily through their actions at CB1 receptor (CB1R), is
implicated in drug relapse. Previous results from our lab demonstrated that in extinguished
cocaine-conditioned animals, evaluated in a conditioned place preference test (CPP), the
administration of AM251, a CB1R antagonist, or ACEA, a CB1R agonist, into the Core of the
Nucleus Accumbens (NAc) abrogated or facilitated restraint stress-induced reinstatement
of cocaine-CPP responses, respectively. In order to compare the involvement of both NAc
compartments, extinguished cocaine-conditioned Wistar rats were microinjected into the
Shell of NAc with ACEA (0.01fmol/side), AM251 (10ug/side) or vehicle, and subsequently
assigned to the following treatments: 1) Stressed Animals (SA): 15 or 30 min-restraint
exposure, depending on the experiment, and 2) Control Animals (CA). The intra-Shell
administration of CB1R antagonist or agonist did not modify the restraint stress-induced
reinstatement of cocaine-CPP responses as previously observed after intra-Core
administration of CB1R ligands. These findings support the hypothesis of the preferential
influence of CB1R within NAc Core, but not Shell, in the reinstatement of cocaine seeking
behavior. Future studies will attempt to identify a possible glutamate dependent
mechanism underpinning the effects of CB1R ligands on the restraint stress-induced
reinstatement of cocaine-CPP responses.
implicated in drug relapse. Previous results from our lab demonstrated that in extinguished
cocaine-conditioned animals, evaluated in a conditioned place preference test (CPP), the
administration of AM251, a CB1R antagonist, or ACEA, a CB1R agonist, into the Core of the
Nucleus Accumbens (NAc) abrogated or facilitated restraint stress-induced reinstatement
of cocaine-CPP responses, respectively. In order to compare the involvement of both NAc
compartments, extinguished cocaine-conditioned Wistar rats were microinjected into the
Shell of NAc with ACEA (0.01fmol/side), AM251 (10ug/side) or vehicle, and subsequently
assigned to the following treatments: 1) Stressed Animals (SA): 15 or 30 min-restraint
exposure, depending on the experiment, and 2) Control Animals (CA). The intra-Shell
administration of CB1R antagonist or agonist did not modify the restraint stress-induced
reinstatement of cocaine-CPP responses as previously observed after intra-Core
administration of CB1R ligands. These findings support the hypothesis of the preferential
influence of CB1R within NAc Core, but not Shell, in the reinstatement of cocaine seeking
behavior. Future studies will attempt to identify a possible glutamate dependent
mechanism underpinning the effects of CB1R ligands on the restraint stress-induced
reinstatement of cocaine-CPP responses.
