A standard numbering scheme for class C β-lactamases

MACK AR; BARNES MD; TARACILA MA; HUJER AM; HUJER KM; CABOT G; FELDGARDEN M; HAFT D; KLIMKE W; VAN DER AKKER F; VILA AJ; SMANIA AM; HAIDER S; PAPP-WALLACE KM; BRADFORD PA; ROSSOLINI GM; DOCQUIER JD; FRERE JM; GALLENI M; HANSON ND; OLIVER A; PLESIAT P; POIREL L; NORDMANN P; PALZKILL TG ; JACOBY GA; BUSH K; BONOMO RA

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2020 vol. 64

0066-4804

nlike the class A and class B β-lactamases, a standardized amino acid residue numbering system for the class C (AmpC) β-lactamases has not been proposed. This lack of standardization can lead to ambiguity when referencing the same residue with different numbers and complicates comparisons between diverse AmpC β-lactamases. Having a standardized numbering system would eliminate this ambiguity, facilitate communication within the field, and greatly simplify comparisons between families of AmpC β-lactamases. Here, we propose a standardized numbering system for AmpC β-lactamases which is based on a multiple sequence alignment of 32 diverse enzymes and accounts for secondary structure of the enzymes. We outline a simple, straightforward, and comprehensive system for assigning amino residue numbers to AmpC β-lactamases that preserves the traditional numbering of key residues (Ser64, Lys67, Tyr150, and Lys315) and defines a means of assigning residue numbers for