A PREVIOUS HISTORY OF REPEATED AMPHETAMINE EXPOSURE MODIFIES BRAIN ANGIOTENSIN II AT1 RECEPTORS FUNCTIONALITY

CASARSA BS; MARINZALDA MA; MARCHESE NA; PAZ MC; VIVAS LM; BAIARDI, G; BREGONZIO C

NEUROSCIENCE

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2015 vol. 307 p. 1 - 1

0306-4522

revious results from our laboratory showed that angiotensin II AT1 receptors (AT1-R) areinvolved in the neuroadaptative changes induced by amphetamine. The aim of the presentwork was to study functional and neurochemical responses to angiotensin II (ANG II)mediated by AT1-R activation in animals previously exposed to amphetamine. For thispurpose male Wistar rats (250-320g) were treated with amphetamine (2.5mg/kg/dayintraperitoneal) or saline for 5 days and implanted with intracerebroventricular (i.c.v.)cannulae. Seven days after the last amphetamine administration the animals received ANG II(400pmol) i.c.v. One group was tested in a free choice paradigm for sodium (2% NaCl) andwater intake and sacrificed for Fos immunoreactivity (Fos-IR) determinations. In a secondgroup of rats, urine and plasma samples were collected for electrolytes and plasma reninactivity determination and then they were sacrificed for Fos immunoreactivit