Allopregnanolone increase in striatal N-methyl-D-aspartic acid evoked [3H]dopamine release is estrogen and progesterone dependent

CABRERA RJ, BREGONZIO C, LACONI M, MAMPEL A

CELLULAR AND MOLECULAR NEUROBIOLOGY.

Año: 2002 vol. 22 p. 445 - 445

0272-4340

p class="abstract">1. The neurosteroids are compounds derived from steroid hormones and synthesized in the nervous system. They can modulate different neurotransmitter pathways. In previous work we demonstrated that progesterone modulates dopamine release induced by the glutamatergic agonist N-methyl-D-aspartic acid (NMDA). 2. The aim of this work was to evaluate a possible modulatory role of the progesterone metabolite allopregnanolone on NMDA-evoked [3H]dopamine release from corpus striatum slices obtained from cycling and ovariectomized female rats. 3. We used a dynamic superfusion method to evaluate the release of [3H]dopamine. Allopregnanolone at 50-600 nM was added to the superfusion buffer (Krebs-Ringer-bicarbonate-glucose. pH 7.4. with constant O2/CO2 gassing). The results are expressed as a percentage over basal [3H]dopamine loaded by the tissue. 4. Allopregnanolone (50 and 100 nM) increased the NMDA-evoked [3H]dopamine release from estrus rats. The remaining doses did not sh