Time-related changes in functional activity and capacitation of chinchilla (Chinchilla lanigera) spermatozoa during in vitro incubation.

PONZIO M.F; BUSSO J.M.; RUIZ R.D.; FIOL DE CUNEO M

Elsevier Science.

Lugar: Gainesville, Florida; Año: 2007 vol. 102 p. 343 - 343

font face="Times-Roman" size="3"> The application of assisted breeding programs for chinchilla, an endangered species, requires detailed knowledge about their gamete physiology. Main purposes of the present study were to examine the timerelated changes during 8 h in vitro incubation in parameters that reflect chinchilla sperm functional activity (including sperm motility, viability, membrane and acrosome integrity), and to determine the incubation time required for achieving in vitro sperm capacitation, evaluated through the quantification of the percentages of sperm that underwent the acrosome reaction in response to progesterone (P, 20 M) or another acrosome reaction inducer the calcium ionophore, A23187 (20 nM). Semen was obtained by electroejaculation,subjected to swim-up and incubated for 0, 2