Noninvasive monitoring of ovarian endocrine activity in the chinchilla (Chinchilla lanigera).

BUSSO JM; PONZIO MF; CHIARAVIGLIO M; FIOL DE CUNEO M,; RUIZ RD

Elsevier Science.

Lugar: Gainesville, Florida; Año: 2007 vol. 150 p. 288 - 288

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt">Reproductive endocrinology information is limited for Chinchilla lanigera; a south American species characterized by extremely long gestation and estrus cycle compared to others rodents. This study was designed to validate a non invasive technique for monitoring ovarian endocrine activity. Animals were exposed indoors to natural photoperiod (31° S - 64° W, Argentina); temperature range: 17-26 °C, with food and water ad libitum. Radiolabeled infusion (n=4): 3H-estradiol (2H-E2) and 14C-progesterone (14C-P4) were injected (i.p). Biochemical validation: HPLC-UV detector was employed to determine natural steroids in urine and fecal extracts and to determine