Resumen:
p style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none" class="MsoNormal">Oxidative stress induced by ciprofloxacin and pyoverdin, a leukotoxic pigment, was studied by comparing their effect in bacteria and leukocytes. Chemiluminescence (CL) assays with lucigenin or luminol were adapted to measure the stimuli of superoxide anion (O2-) and other reactive species of oxygen (ROS) in bacteria. Ciprofloxacin principally induced the production of O2- in the three species studied: Staphylococcus aureus, Enterococcus faecalis and Escherichia coli. Lucigenin CL assay showed high oxidative stress in S. aureus due to its low superoxide dismutase (SOD) activity, whereas E. coli exhibited important SOD activity, responsible for little production of O2- in absence or presence o