MASINI ANA MARIA DE LAS MERCEDES
Artículos
Título:
Bacterial lipopolisaccharide stimulates the TSH dependent thyroglobulin gene expression at transcriptional level by involving TTF-1 and Pax8 transription factors
Autor/es:
MARÍA L. VÉLEZ, EUGENIA COSTAMAGNA, EDNA T. KIMURA, LAURA FOZZATTI, CLAUDIA G, PELLIZAS, MARÍA M. MONTESINOS, ARIEL M. LUCERO, ALDO H. COLEONI, PILAR SANTISTEBAN, ANA M. MASINI-REPISO
Editorial:
The Endocrine Society
Referencias:
Lugar: Bethesda, MD, USA; Año: 2006 vol. 147 p. 3260 - 3260
Resumen:
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The bacterial lipopolysaccharide (LPS) is a biological activator that induces multiple gene expression in several cell types. LPS has been proposed as an etiopathogenic agent in autoimmune diseases. However, whether LPS affects the expression of autoantigens has not been explored. Thyroglobulin (TG) is a key protein in the thyroid hormonogenesis and one of the major thyroid autoantigens. This study aimed to analyze the action of LPS on the TG gene expression in FRTL-5 thyroid cells. We demonstrated that LPS increased the TSH-induced TG protein and mRNA level. Evidence that the effect of LPS was exerted at transcriptional level was obtained by transfecting the minimal TG promoter. The C element of the TG promoter, which contains sequences for Pax8 and TTF-1 binding, is essential for the TG promoter full expression under TSH stimulation. We observed that the transcriptional activity of a construct containing five tandem repeats of the C site was