Selection of a high quality subpopulation of frozen thawed bovine spermatozoa by filtration in a sephadex column. An assessment of functional effects of progesterone.

VINCENTI LM; FIOL DE CUNEO M; RUIZ RD; MARTINI AC; PONCE AA

Journal of experimental animal science

Urban and Fischer Verlag

Año: 2002 vol. 42 p. 93 - 93

nbsp; It is accepted  that cryopreservation exerts deleterious effects on functional characteristics of mammalian spermatozoa. Conventional procedures for processing frozen-thawed gametes, such as centrifugation, produce additional damage. In the present work, we investigated the efficacy of processing bovine cryopreserved semen by filtration in a Sephadex column (SF group) or by washing by centrifugation (100 g, 10 min, twice) (W group); the results obtained from both procedures were compared to untreated samples (C group). The effects of in vitro addition of progesterone (10