Improving the predictive value of the hypoosmotic swelling test in humans.

MARTINI AC; MOLINA RI; ESTOFÁN D; TISSERA A; RUIZ RD; FIOL DE CUNEO M

Elsevier Science

Año: 2006 vol. 85 p. 1840 - 1840

span lang="en" style="FONT-FAMILY: "Times New Roman"; FONT-SIZE: 12pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: ES; mso-bidi-language: AR-SA">   We evaluated, employing a combined hypoosmotic swelling-eosin (HOS-E) technique in human semen samples, the frequency of dead swollen spermatozoa (dHOS) after 10 and 30 min incubation, the correlation between total HOS-reactive (tHOS) and viable HOS-reactive (vHOS) spermatozoa with other seminal parameters and the possibility that dead spermatozoa react to HOS. We obtained the following results: a) some dead spermatozoa swell under hypoosmotic conditions and b) HOS-E results correlate strongly with other seminal parameters and we recommended to perform HOS after 10 min incubation because: a) the increase in the incubation time enhances the