NATES SILVIA VIVIANA
Artículos
Título:
Evidence of closely related picobirnavirus strains circulating in humans and pigs in Argentina.
Autor/es:
MIGUEL O. GIORDANO; LAURA C. MARTÍNEZ; GISELA MASACHESSI; PATRICIA A. BARRIL; LEONARDO FERREYRA; MARÍA B. ISA; MILDRE C. VALLE; PABLO U. MASSARI; SILVIA V. NATES
Editorial:
W B SAUNDERS CO LTD
Referencias:
Lugar: Londres; Año: 2011 vol. 62 p. 45 - 45
Resumen:
ackground: On the basis of the published literature, it is still difficult to draw conclusions as to whether picobirnavirus (PBV) circulation is influenced by host species restriction. Objective: To provide data regarding the genetic relatedness between porcine and human PBV strains present in Argentina as a means of defining the host range and epidemiology of these viruses. Methods: Fecal specimens (n Z 74) collected from kidney transplant patients (n Z 55) and piglets (n Z 19) were analyzed by RT-PCR using primers designed to amplify the porcine PBV genomic segment 2. Amplified sequences were further examined phylogenetically. Results: By RT-PCR amplification 14 of 74 samples rendered amplicons of the expected 282 base pair size (8 detected from humans and 6 from pigs). Eleven amplicons (5 from humans and 6 from pigs) were selected for sequencing and subjected to phylogenetic analysis. The eleven amplicons revealed similarities between human and porcine viral sequences th