Culture amplification in human colon adenocarcinoma cell line (CaCo-2) combined with an ELISA as a supplementary assay for accurate diagnosis of rotavirus.

CUMINO AC, GIORDANO MO, MARTÍNEZ LC, MEDEOT SI, PAVAN JV, YUDOWSKY S, ISA MB, DEPETRIS AR, NATES SV.

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1998 vol. 76 p. 81 - 81

Virol Methods. 1998 Dec;76(1-2):81-5. Culture amplification in human colon adenocarcinoma cell line (CaCo-2)combined with an ELISA as a supplementary assay for accurate diagnosis ofrotavirus. Cumino AC1, Giordano MO, Martínez LC, Medeot SI, Pavan JV, Yudowsky S, Isa MB, Depetris AR, Nates SV. Author information Abstract Culture amplification in colon adenocarcinoma cell line (CaCo-2) combined with enzyme immunoassay (Pathfinder ELISA) was developed as a supplementary tool for rotavirus diagnosis. One hundred and thirty stools in which results by polyacrylamide gel electrophoresis (PAGE) were in agreement with those obtained by ELISA were amplified in the CaCo-2 cell line. After the first passage 100% specimens were revealed as positive by ELISA. This result was confirmed by PAGE and direct electron microscopy (EM) which increased the rates of rotavirus detection up to 100% after the third and fifth cell passages, respectively. All of the amplified negative stools were confirmed