Artículos
Título:
Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay.
Autor/es:
GABRIELA CARRUYO; GUANIRI MATEU; LAURA C. MARTÍNEZ; FLOR H. PUJOL; SILVIA V. NATES; FERDINANDO LIPRANDI; JUAN E. LUDERT
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 2008 vol. 46 p. 2402 - 2402
Resumen:
he molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine
PBV described so far. Analysis by RT-PCR and polyacrylamide gel
electrophoresis of fecal samples collected in Venezuela and Argentina
showed that PBV circulate at high frequencies in piglets.