Evaluation of Cytotoxicity and Genotoxicity of Acacia aroma Leaf Extracts

MATTANA CLAUDIA; CANGIANO M; ALCARÁZ LUCIA; SOSA A.; ESCOBAR FRANCO MATÍAS; SABINI MARÍA CAROLA; SABINI LILIANA INÉS; LACIAR ANALÍA

THESCIENTIFICWORLD LTD

Lugar: New York; Año: 2014 p. 1 - 1

bstract Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100-5000 µg/mL of HAE and EE showed that 500 µg/mL and 100 µg/ mL were the maximum non-cytotoxic concentration, respectively. The CC50 was 658µg/mL for EE and 1020µg/mL for HAE. The genotoxicity was tested by the single-cell gel electrophoresis comet assay. The results obtained in the evaluation of DNA cellular damage exposed to varieted concentrations the HAE showed not significantly genotoxic effect at range of 1-20 mg/mL. The EE at 20 mg/mL showed moderate genotoxic effect related to the increase of the DNA percentage contain in tail the comet, DNA was clasificated in category 2. At concentrations below