BECERRA MARIA CECILIA
Artículos
Título:
ANTIBACTERIAL ACTIVITY OF A COMBINATION OF CYSTEINE AND CIPROFLOXACIN AND ITS RELATIONSHIP WITH THE GENERATION OF OXIDATIVE STRESS IN EXTENDED-SPECTRUM BETALACTAMASE-PRODUCING ESCHERICHIA COLI STRAINS
Autor/es:
MARTÍNEZ SR; BONGIOVANNI ME; PIERSIGILLI A; ALBESA I; BECERRA MC ; BECERRA M C
Revista:
Research Journal of Life science, bioinformatics, pharmaceutical and chemical sciences
Editorial:
Life Science Informatics Publications
Referencias:
Año: 2018 vol. 4 p. 455 - 455
ISSN:
2454.6348
Resumen:
he aim of the present work was to evaluate whether L-cysteine enhances theantibiotic susceptibility of extended-spectrum beta-lactamase (ESBL) Escherichia coli strains to ciprofloxacin and to identify the role of Reactive oxygen species (ROS) in the antimicrobial activity.The combined ef5fect of L-cysteine and ciprofloxacin was investigated by the macrodilution broth method and chemiluminescence assay. ESBL 1 and ESBL 2 strains presented a growth inhibition when they were incubated with 0.4 or 0.2 mM of cysteine, respectively compared to the control without antibiotic. Both strains at sub-inhibitory concentration of ciprofloxacin were combined withcysteine, and a clear growth inhibition respect to the control was observed. An increase of 68% in ROS occurred respect to the control when ESBL 1 was treated with a combination of ciprofloxacin and cysteine, while for ESBL 2 there was a rice of 127 %. The combination of ciprofloxacin with cysteine had the capacity to undergo redox cycling a