Estrogen receptor a regulates the tumoral suppressor PTEN to modulate pituitary cell growth?

PEREZ P.; PETITI J.P.; PICECH F.; GUIDO C.; SOSA L.; GRONDONA E.; MUKDSI J. H.; DE PAUL A.L.; TORRES A.I.; GUTIÉRREZ S.

JOURNAL OF CELLULAR PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2017 vol. 233

0021-9541

n this study, we focused on ERβ regulation in the adenohypophysis under different estrogenic milieu, by analyzing whether ER modulates the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) expression and its subcellular localization on anterior pituitary glands from Wistar rats andGH3 lactosomatotroph cells that over-expressed ERβ. ERβ was regulated in a cyclic manner, and underwent dynamic changes throughout the estrous cycle, with decreased ERβ+ cells in estrus and under E2 treatment, but increased in ovariectomized rats. In addition, the ERα/β ratio increased in estrus and under E2 stimulation, but decreased in ovariectomized rats. Double immunofluorescencerevealed that lactotroph and somatotroph ERβ+ were significantly decreased in estrus. Also, variations in the PTEN expression was observed, which was diminished with high E2 conditions but augmented with low E2 milieu. The subcellular localization of this phosphatase was cell cycl