The ERalpha; membrane pool modulates the proliferation of pituitary tumours

SOSA, LILIANA DEL V; PETITI, JUAN P; PICECH, FLORENCIA; CHUMPEN, SABRINA; NICOLA, JUAN P; PEREZ, PABLO; DE PAUL, ANA; VALDEZ-TAUBAS, JAVIER; GUTIERREZ, SILVINA; TORRES, ALICIA I

JOURNAL OF ENDOCRINOLOGY

BIOSCIENTIFICA LTD

Año: 2018

0022-0795

he molecular mechanisms underlying the ERa nuclear/cytoplasmic pool that modulates pituitary cell proliferation have been widely described, but it is still not clear how ERa is targeted to the plasma membrane. The aim of this study was to analyse ERa palmitoylation and the plasma membrane ERa (mERa) pool, and their participation in E2-triggered membrane-initiated signalling in normal and pituitary tumour cell growth. Cell cultures were prepared from anterior pituitaries of female Wistar rats and tumour GH3 cells, and treated with 10nM of estradiol (E2). The basal expression of ERa was higher in tumour GH3 than in normal pituitary cells. Full-length palmitoylated ERa was observed in normal and pituitary tumour cells, demonstrating that E2 stimulation increased both, ERa in plasma membrane and ERa and caveolin-1 interaction after short-term-treatment. In addition, the Dhhc7 and Dhhc21 palmitoylases were negatively regulated after sustained stimulation of E2 for 3h. Although the uptake o