NÚÑEZ MONTOYA SUSANA CAROLINA
Artículos
Título:
In vitro and in vivo cytogenotoxic effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.
Autor/es:
L. N. CARIDDI; M. C. SABINI; F. M. ESCOBAR; R. BACCHETTI; I. MONTIRONI; C. MERCKIS; B. REINOSO; NÚÑEZ MONYOYA S.C.; S. M. ZANON; L. R. COMINI; L. I. SABINI
Revista:
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
Editorial:
HINDAWI PUBLISHING CORPORATION
Referencias:
Lugar: New York; Año: 2015 vol. 2015 p. 1 - 1
ISSN:
1110-7243
Resumen:
n this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653𝜇g/mL) and MTT (CC50 = 588𝜇g/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquer