Potent inhibition of Western equine encephalitis virus by a fraction rich in flavonoids and phenolic acids obtained from Achyrocline satureioides

SABINI, MARÍA CAROLA; CARIDDI, LAURA NOELIA; ESCOBAR, FRANCO MATÍAS; MAÑAS, FERNANDO; COMINI, LAURA; IGLESIAS, DELVIS; LARRAURI, MARIANA; NÚÑEZ MONTOYA, SUSANA CAROLINA; SERENO, JOSÉ; CONTIGIANI, MARTA SILVIA; CANTERO, JUAN JOSÉ; SABINI, LILIANA INÉS

REVISTA BRASILEIRA DE FARMACOGNOSIA

JOHN BENJAMINS PUBLISHING COMPANY

Año: 2016 vol. 26 p. 571 - 571

0102-695X

chyrocline satureioides (Lam.) DC. Asteraceae, ?marcela del campo´, possess several pharmacological properties. Previously we reported antiviral activity of an aqueous extract of A. satureioides against an alphavirus, Western Equine Encephalitis virus. Alphaviruses are highly virulent pathogens which cause encephalitis in humans and equines. There are no effective antiviral to treat its infections. The aim of this study was to evaluate in vitrocytotoxic and antiviral activities against Western Equine Encephalitis virus of five water extract chromatographic fractions from A. satureioides and identify the main compounds of the bioactive fraction. Also, it was to assess in vivo cytogenotoxic ability of the active fraction. Cytotoxicity studies revealed low toxicity of the most of fractions in Vero and in equine peripheral blood mononuclear cells. Antiviral studies showed that the water crude extract ? Sephadex LH 20 ? fraction 3 MeOH?H2O (Fraction 3) was active against Western Equine Enc