Experimental poisoning by Heterophyllaea pustulata Hook. f. (Rubiaceae) in goats. Clinical, biochemical and toxicological aspects

MICHELOUD, JUAN FRANCISCO; AGUIRRE, LAURA S.; MARIONI, JULIANA; MUGAS, MARIA LAURA; CABRERA, JOSÉ LUIS; MARTINEZ, OLGA GLADYS; GALLARDO, SILVINA CAROLA; GIMENO, EDUARDO JUAN; NÚÑEZ-MONTOYA, SUSANA CAROLINA

TOXICON

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2019 vol. 165 p. 56 - 56

0041-0101

he aim of this study was to investigate the clinical, biochemical and toxicological findings of the experimentally poisoning induced by Heterophyllaea pustulata in goats. Ten healthy adult female Saanen breed goats were used in the experiment. The goats were randomly assigned to two groups of five individuals: control and experimental group (CG and EG). Both groups were kept in the same enclosure devoid of shade for 8 h daily. The EG received only H. pustulata samples (leaves and thin steam) and water ad libitum. The CG received lucerne hay. Blood samples were taken at different times after oral administration of vegetal samples, and level of hepatic enzymes, total bilirubin, conjugated and non-conjugated bilirubin was measured, together with the detection of anthraquinones (AQs) and phylloerythrin by High Performance Liquid Chromatography with Diode-Array Detector and Mass Spectrometry with Electron Spray Ionization and Quadrupole Time Of Fly analysis. At the same time, skin biopsy s