Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus

ZYGADLO, JULIO A.

BIORESOURCE TECHNOLOGY

ELSEVIER SCI LTD

Año: 2019 vol. 289

0960-8524

leurotus sapidus monokaryotic strains (Mk) were screened as a novel source of mycelia to valorize rice straw(RS), rice husks (RH) and sunflower seed hulls (SSH) into value-added products through solid-state fermentation(SSF). P. sapidus Dk3174 basidiospores were cultured in the presence of Remazol Brillant Blue R for strainselection, revealing the ligninolytic ability of emerging colonies. Further screening demonstrated the intraspecificvariability in dye degradation and enzyme production of 63 strains. Growth rate, biomass and enzymeproduction in plates containing RS, RH or SSH pointed at MkP6 as a suitable strain for pilot-scale SSF. MkP6presented a similar laccase profile as the parental Dk3174, being greater in pasteurized substrates (300?1200 U/Kg) than in sterilized substrates (30?250 U/Kg). Peroxidase represented 25% of the total ligninolytic activitymeasured. The SSH fermented biomass with MkP6 obtained good yields of nanocellulose (67%) and the sacchariderelease for ethanol p