Novel alleles linked to brown rust resistance in sugarcane

CHAVES, SOLANA; OSTENGO, SANTIAGO; BERTANI, ROMINA PRISCILA; PEÑA MALAVERA, ANDREA NATALIA; CUENYA, MARÍA INÉS; FILIPPONE, MARÍA PAULA; CASTAGNARO, ATILIO PEDRO; BALZARINI, MÓNICA GRACIELA; RACEDO, JOSEFINA

PLANT PATHOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2022

0032-0862

ugarcane brown rust, caused by Puccinia melanocephala, is a severe foliar disease that occurs in almost all countries where sugarcane is grown. The main control strategy is the use of resistant cultivars. The aim of this work was to identify molecular markers linked to genomic regions associated with a novel brown rust resistance source in sugarcane. An F1 progeny of 300 clones was obtained from a cross between TUC 00–36 and RA 87–3, highly susceptible and highly resistant to brown rust, respectively. A total of 60 F1 clones with extreme phenotype, either highly susceptible or highly resistant to brown rust, were selected. This “pooled tail” population was tested for reactions to brown rust under natural infection in the field during two crop seasons and under artificial infection in the greenhouse. Whole-genome profiling was performed by DArT-seq technology. Phenotypic data under both conditions and 23,299 single-nucleotide polymorphisms (SNPs) obtained from genotyping were analysed