The metabolism of phospholipids oscillates rhythmically in cultures of fibroblasts and is regulated by the clock protein period 1

VALERIA PAOLA CARLINI; SEBASTIAN MARQUEZ; VALERIA CARLINI; PILAR CRESPO; GARBARINO PICO EDUARDO; RUBEN BALER; BEATRIZ CAPUTO; MARIO GUIDO

FASEB JOURNAL

Año: 2004 vol. 18 p. 519 - 519

0892-6638

font face="TimesNewRoman" color="#ff0000"> ABSTRACT The mammalian circadian timing system is composed of countless cell oscillators distributed throughout the body and central pacemakers regulating temporal physiology and behavior. Peripheral clocks display circadian rhythms in gene expression both in vivo and in culture. We examined the biosynthesis of phospholipids as well as the expression of the clock gene period 1 (Per1) and its potential involvement in the regulation of the phospholipid metabolism in cultured quiescent NIH 3T3 cells synchronized by a 2 h serum shock. A 30 min pulse of radiolabeled precursor was given at phases ranging from 0.5 to 62 h after serum treatment. We observed a daily rhythm in the phospholipid labeling that persisted at least f