Artículos
Título:
Molecular detection of SARS-CoV-2 in Argentina: evaluation of alternative diagnostic tools for the decentralization of the diagnosis
Autor/es:
CASTRO, GONZALO MANUEL; SICILIA, PAOLA; GIEROTTO, ROBERTINO; SOSA, JULIETA; CASTELLARO, ANDRÉS MARCOS; BARBÁS, MARÍA GABRIELA; PISANO, MARÍA BELÉN; RÉ, VIVIANA E.
Revista:
REVISTA ARGENTINA DE MICROBIOLOGÃA
Editorial:
ASOCIACION ARGENTINA MICROBIOLOGIA
Referencias:
Lugar: Buenos Aires; Año: 2023
Resumen:
he rocketing number of COVID-19 cases highlighted the critical role that diagnostictests play in medical and public health decision-making to contain and mitigate theSARS-CoV-2 pandemic. This study reports the evaluation and implementation ofdifferent tests for the molecular detection of SARS-CoV-2 in the central region ofArgentina. We evaluated 3 real time RT-PCR kits (GeneFinder COVID-19 Plus RealAmpKit, DisCoVery SARS-CoV-2 RT-PCR Detection kit and WGene SARS-CoV-2 RT Detection),2 nucleic acid extraction methods [MagaBio plus Virus DNA/RNA Purification Kit II(BioFlux), 35-minute vs. 9-minute], a pre-analytical reagent (FlashPrep®) and 2isothermal amplification tests (Neokit Plus and ELA CHEMSTRIP®). The order accordingto the best performance of the 3 real-time RT-PCR kits evaluated was:DisCoVery>GeneFinderTM>WGene. The 2 RNA extraction methods showed similargood results: MagaBio plus Virus RNA Purification Kit II (BioFlux) 9-min was selecteddue to its faster performance. FlashPrep®