GONZALO MANUEL CASTRO
Artículos
Título:
Hepatitis A virus monitoring in wastewater: A complementary tool to clinical surveillance
Autor/es:
FANTILLI, ANABELLA; COLA, GUADALUPE DI; CASTRO, GONZALO; SICILIA, PAOLA; CACHI, ARIANA MARIELA; DE LOS ÁNGELES MARINZALDA, MARÍA; IBARRA, GUSTAVO; LÓPEZ, LAURA; VALDUVINO, CELINA; BARBÁS, GABRIELA; NATES, SILVIA; MASACHESSI, GISELA; PISANO, MARÍA BELÉN; RÉ, VIVIANA
Revista:
WATER RESEARCH
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2023 vol. 241
ISSN:
0043-1354
Resumen:
onitoring wastewater is an effective tool for tracking information on trends of enteric viral dissemination. Thisstudy aimed to perform molecular detection and genetic characterization of HAV in wastewater and to correlatethe results with those obtained from clinical surveillance.Wastewater samples (n=811) of the second most populous city in Argentina were collected from the mainwastewater treatment plant (BG-WWTP, n=261), and at 7 local neighborhood collector sewers (LNCS, n=550)during 2017–2022. Clinical samples of acute hepatitis A cases (HA, n=54) were also analyzed. HAV moleculardetection was performed by real time RT-PCR, and genetic characterization by RT-Nested PCR, sequencing andphylogenetic analysis.RNA-HAV was detected in sewage samples throughout the entire period studied, and detection frequenciesvaried according to the location and year (2.9% - 56.5%). In BG-WWTP, 23% of the samples were RNA-HAV+.The highest detection rates were in 2017 (30.0%), 2018 (41.7%) and 2022 (56