Effect of fluoride on bone and growth plate cartilage

LOMBARTE, MERCEDES; FINA, BRENDA L.; BRUN, LUCAS R.; ROMA, STELLA MARIS; RIGALLI, ALFREDO; DI LORETO, VERÓNICA E

Journal of Environmental Science and Health, Part C: Toxicology and Carcinogenesis

Taylor and Francis Ltd.

Año: 2021 vol. 39 p. 388 - 388

2689-6583

he use of fluoride (F) for therapeutic purposes is controversial and its toxicity is a health problem. The aim of this study was to evaluate the effect of F on endochondral ossification in growing rats. Twenty-four rats of 21 days were divided into 4 groups which received 0, 20, 40 or 80 μmol F/100 g body weight/day for 30 days, through an orogastric tube. Histological evaluation of growth plate cartilage (GPC) and primary and secondary bone were analyzed on sections of the metaphysis of tibias. Total thickness of the GPC (GPC.Th), thickness of resting zone (RZ.Th), proliferative zone (PZ.Th) and hypertrophic zone (HZ.Th); bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and apoptosis by the TUNEL were measured. A hyperplasia of the proliferative zone and a significant increase in PZ.Th with 40 and 80 μmol F without changes in GPC.Th were found. In the secondary trabecular bone, presence of immature trabeculae, perit