AMÉ MARÍA VALERIA
Congresos y reuniones científicas
Título:
Uptake, detoxication and accumulation of Microcystin-LR in shrimps (Palaemonetes argentinus).
Autor/es:
GALANTI, LUCAS NICOLáS; AMÉ, MARÍA VALERIA; WUNDERLIN, DANIEL ALBERTO
Reunión:
Congreso; http://berlin.setac.eu/scientific_programme/download_the_abstracts_book/?contentid=582&pr_id=403&last=435; 2012
Resumen:
Microcystins (MCs) are cyclic heptapeptide produced by cianobacteria. There are over 100 different MCs congeners reported up to now, having different toxicity. MC-LR is
by far the most studied and ubiquitous toxin, with know hepatotoxic effects, inhibition of protein-phosphatases 1 and 2-A, which promotes cancer and other diseases.
MC-LR has been found in water bodies worldwide. Still today, little is known on how this toxin is transferred through the food web from water or cianobacteria. Our main
goal was evaluating the uptake of soluble MC-LR by small shrimps (P. argentinus) that share the same habitat with cianobacteria. Additionally, we evaluated the detoxication
period after initial uptake as well as the activity of several enzymes involved in detoxicatio Glutathion-S-Tranferase or antioxidant defence system Catalase, Glutathion
Reductase and Glutathion Peroxidase. So, we look to evaluate both bioaccumulation and detoxication of MC-LR in shrimps as the first step in the transference of MCs
within the aquatic food web, considering that shrimps are predated by fish, which are then captured by other fish or used as food by humans. Analysis of MC-LR in water
and shrimps was done by HPLC-PDA-ESI-MS (either triple quadrupole or Q-TOF). Shrimps were exposed to soluble MC-LR (50 ìg L-1) during 3 days and then translated
to new tanks without MCs for additional three days. Crustaceans presented bioaccumulation after 3 days exposure (0.74 ± 0.15 ìg MC-LR g-1 shrimp). The amount of MCLR
was reduced to 0.18 ± 0.01 ìg MC-LR g-1 shrimp after three days detoxication (75% MC-LR removal during the detoxication period). The activity of studied enzymes
remained almost unchanged during the exposure period but Glutathion-S-Tranferase and Glutathion Reductase were induced (activated) during the detoxication period. These
preliminary results suggest that MC-LR can be bioaccumulated and eliminated in shimps. On the other hand both detoxication and antioxidant enzymes seem to be repressed
during the exposure but stimulated after exposure, enabling detoxication. Our current results suggest that the uptake, bioaccumulation and detoxication process is complex
and demonstrate that MC-LR is bioaccumulated by shrimps upon exposure to environmental relevant amounts of this toxin. Furthermore, detoxication enzymes seem to be
inhibited during the exposure period. However, detoxication enzymes are activated as soon as the exposure stops, enabling 75% removal after three days.
by far the most studied and ubiquitous toxin, with know hepatotoxic effects, inhibition of protein-phosphatases 1 and 2-A, which promotes cancer and other diseases.
MC-LR has been found in water bodies worldwide. Still today, little is known on how this toxin is transferred through the food web from water or cianobacteria. Our main
goal was evaluating the uptake of soluble MC-LR by small shrimps (P. argentinus) that share the same habitat with cianobacteria. Additionally, we evaluated the detoxication
period after initial uptake as well as the activity of several enzymes involved in detoxicatio Glutathion-S-Tranferase or antioxidant defence system Catalase, Glutathion
Reductase and Glutathion Peroxidase. So, we look to evaluate both bioaccumulation and detoxication of MC-LR in shrimps as the first step in the transference of MCs
within the aquatic food web, considering that shrimps are predated by fish, which are then captured by other fish or used as food by humans. Analysis of MC-LR in water
and shrimps was done by HPLC-PDA-ESI-MS (either triple quadrupole or Q-TOF). Shrimps were exposed to soluble MC-LR (50 ìg L-1) during 3 days and then translated
to new tanks without MCs for additional three days. Crustaceans presented bioaccumulation after 3 days exposure (0.74 ± 0.15 ìg MC-LR g-1 shrimp). The amount of MCLR
was reduced to 0.18 ± 0.01 ìg MC-LR g-1 shrimp after three days detoxication (75% MC-LR removal during the detoxication period). The activity of studied enzymes
remained almost unchanged during the exposure period but Glutathion-S-Tranferase and Glutathion Reductase were induced (activated) during the detoxication period. These
preliminary results suggest that MC-LR can be bioaccumulated and eliminated in shimps. On the other hand both detoxication and antioxidant enzymes seem to be repressed
during the exposure but stimulated after exposure, enabling detoxication. Our current results suggest that the uptake, bioaccumulation and detoxication process is complex
and demonstrate that MC-LR is bioaccumulated by shrimps upon exposure to environmental relevant amounts of this toxin. Furthermore, detoxication enzymes seem to be
inhibited during the exposure period. However, detoxication enzymes are activated as soon as the exposure stops, enabling 75% removal after three days.
