Physicochemical Caracterization and Biological Properties from Novel Biogenic Hydroxyapatita

BABOT E; MISSANA L; BLANCO M. C.; JUÁREZ J; PASTORINO N; CATALÁN M; WINIK B

Mar del Plata

Congreso; XL Reunión Anual de la Sociedad Argentina de Investigación Odontológica; 2007

Biogenic hydroxyapatita (BHA) is an important medical and dental biomaterial, from many sources like seaweed, coralline and echinoderms. The ecosystem could be affected negativelly by their industrial production. In our lab had been synthesized a new HA from a gastropod that it is a renewable natural resource. The aim os this work was to establish the physicochemical features and biological properties from this new HA. The bone marrow from rat tibia was used for biological test. The chemical composition and crystallinity from this biomaterial was study by powder X ray diffraction (XRD) and Fourier trasnformed infrared sprectroscopy (FT-IR). Its morphologycal features were studied by scanning electron microscopy ( SEM). For a biological test, the new HA where implanted into the bone marrow of rat tibia in order to evaluated its biocompatibility. Animals were euthanized at 7 and 21 days after biomaterial implantation. DRX showed that the obtained biomaterial, corresponds to highly purified hydroxyapatita as well as crystalline, with two preferential orientations. The analysis FT-IR showed high intensity bands, that were a feature observed in phosphate groups. And this is a characteristics from HAP. Moreover, it were found few peaks of low intensity, which were related to residual carbonate groups from the starting material. The SEM demonstrate a crystalline morphology. From light microscopical studies, it was found an important amount of new bone around the new material from 7 days. At any study periods were observed infection, mortallity or implant movility. The results obtained from DRX, FT-IR and MEB studies were agree with a highly pure and crystalline HA. At last, the biological test showed biocompatibility and osteogenecity. From these results, it is able to begin the biological evaluation as bone replacement material.