ANTIOXIDANT COMPOUNDS OF ACHYROCLINE SATUREIOIDES INHIBIT HERPES SUIS TYPE 1 VIRUS

MENIS CANDELA FLORENCIA; SABINI MARÍA CAROLA; ESCOBAR FRANCO MATÍAS; TORRES CRISTINA VANESA; CARIDDI LAURA NOELIA; COMINI LAURA; SABINI LILIANA INÉS

TAFI DEL VALLE

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

ASOCIACION DE BIOLOGIA DE TUCUMAN

ANTIOXIDANT COMPOUNDS OF ACHYROCLINE SATUREIOIDES INHIBIT HERPES SUIS TYPE 1 VIRUSMenis Candela F1, Sabini MC1, Escobar F1, Torres C1, Cariddi N1, Comini L2, Sabini L11Dpto Mic e Inm, CsEFQyNat, UNRC. Ruta 36 Km 601. Río IV, Cba, Argentina. 2 Farmacognosia, Dpto. Farmacia., UNC. Córdoba. E-mail: csabini@exa.unrc.edu.ar.Herpesviruses represent a high impact health problem. Herpes suis type 1 virus causes Aujeszky´s disease and it results in significant economic losses to swine production. Medicinal plants can be useful to solve this problem. Achyrocline satureioides "marcela del campo" has several medicinal properties such as antimicrobial, immunomodulatory, antiviral. Phytochemical analysis of A. satureioides cold aqueous extract indicated the presence of luteolin (L), quercetin (Q), chlorogenic acid (CHLA) and caffeic acid (CA). The purpose of this study was to evaluate the antiviral action in vitro of the compounds (C) of A. satureioides against Herpes suis type 1 virus.Cytotoxicity was assessed on Vero cells by Uptake of Neutral Red (UNR) and MTT reduction assays. Then, the antiviral activity of C in all viral replication cycle was evaluated by plaque reduction assay. Further, the action stage in the replication cycle was determined. Finally, the selectivity index (SI) was calculated. Cytotoxicity studies revealed low toxicity of all C. Antiviral ability studies indicated that H. suis type 1 virus was inhibited in 66% by L at 15 µg/ml. In contrast, CA, Q and, CHLA were not active against the virus. Action stage study indicated that L exerts its action in the post-viral adsorption and penetration stage. Selectivity indices of L were: 105.3 (NRU) and 72.6 (MTT). In conclusion, L showed selective and strong inhibitory ability against H. suis type 1 virus and it may be useful for herpes virus infections treatment.