EGUÍLAZ RUBIO MARCOS
Congresos y reuniones científicas
Título:
Bienzymatic glucose biosensor based on the use of multi-walled carbon nanotubes non-covalently functionalized with cytochrome c
Autor/es:
SOLEDAD BOLLO; CONSTANZA VENEGAS; GUSTAVO A. RIVAS; MARCOS EGUÍLAZ
Lugar:
La Haya
Reunión:
Congreso; 67th Meeting of the International Society of Electrochemistry; 2016
Resumen:
We report for the first timethe use of glassy carbon electrodes (GCE)modified by drop-coatingwithmulti-walled carbon nanotubes (MWCNTs) dispersed in cytochrome c as platformfor the construction of bienzymatic biosensors, using the glycoenzyme glucose oxidase (GOx) as model. The sensitivity of the resulting biosensor at −0.050 V towards glucose using hydroquinone (H2Q) as redox mediator was (96 ± 2) μA M−1, with a linear
range between 1.00 × 10−4 M and 1.00 × 10−3 M and a detection limit of 8 μM. The biosensor demonstrated to be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability ofMWCNTs-Cyt c dispersion and the intimate interaction between Cyt c, MWCNTs and GOx, allowed the construction of an efficient glucose biosensor successfully used to determine glucose in commercial beverages.
range between 1.00 × 10−4 M and 1.00 × 10−3 M and a detection limit of 8 μM. The biosensor demonstrated to be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability ofMWCNTs-Cyt c dispersion and the intimate interaction between Cyt c, MWCNTs and GOx, allowed the construction of an efficient glucose biosensor successfully used to determine glucose in commercial beverages.
