COMBINED METFORMIN AND NARINGIN REVERSES THE METABOLIC ALTERATIONS OF THE NONALCOHOLIC FATTY LIVER DISEASE IN EXPERIMENTAL METABOLIC SYNDROME

MARÍA AGUSTINA RIZZI; TAMARA MAGALÍ MAZO; MARÍA EUGENIA PASQUALINI; NORI TOLOSA DE TALAMONI; ALERIA ANDREA RODRÍGUEZ

Mar de Plata

Congreso; ANNUAL MEETING OF BIOSCIENCE SOCIETIES 2022; 2022

Sociedad argentina de investigación clinica

The fructose rich diet (FRD) causes metabolic alterations that resemblemetabolic syndrome (MS), which are associated with non-alcoholicfatty liver disease (NAFLD). Metformin (Met), is a medicineof first line for the treatment of insulin resistance associated withMS. However, it presents adverse reactions and side effects. Naringin(NAR) is a flavonoid with antioxidant, antiapoptotic and anti-inflammatoryproperties. The aim of this work was to know the effectof Met+NAR on the metabolic systemic variables and histologicalalterations in the liver of animals with MS. Male Wistar rats were dividedin 5 groups: 1) controls; 2) rats fed FRD 10% (w/v) in drinkingwater for 60 days; 3) FRD rats treated with Met (100 mg/kg b.w.);4) FRD rats treated with NAR (40 mg/kg b.w.); 5) FRD rats treatedwith Met+NAR. Treatments started from day 21 of FRD administration.Body weight, waist circumference and biochemical parameterswere measured. The adiposity and hepatosomatic index (HSI),and histomorphometric studies were accomplished in liver. ANOVA/Bonferroni was used for statistical analysis. The body weight andwaist circumference s were significantly higher in FRD rats comparedto those from the control rats, while all treatments decreasedwaist circumference. Adiposity and HSI increased in FRD rats ascompared to those from control rats; however, they were reversedwith Met+NAR. The increase in the serum values of triglycerides andAST of FRD rats was completely abrogated by NAR and Met+NARtreatments. FRD rats showed ballooning of hepatocytes with steatosisand dilatation of the portal space and fibrosis. Only the combinedtreatment reversed these alterations. In conclusion, FRD causessystemic metabolic changes, and body and histological alterations.Since the combined treatment reverses the biochemical parametersand histological alterations as well as the liver fibrosis, it constitutesa new therapeutic alternative for NAFLD.