Congresos y reuniones científicas
Título:
Alterations in frontal cortex synaptosomes from rats with experimental autoimmune encephalomyelitis
Autor/es:
CHANADAY NL; VILCAES AA; DE PAUL A; TORRES A; DEGANO AL; ROTH GA
Lugar:
Huerta Grande, Córdoba, Argentina
Reunión:
Congreso; XXVIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2013
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencia (SAN)
Resumen:
Experimental autoimmune encephalomyelitis (EAE) is an animal model of the
human disease multiple sclerosis. Both are inflammatory demyelinating
pathologies of the central nervous system associated with motor, sensory, and
cognitive deficits. In the last years gray matter damage has increasingly received
attention as they may contribute to disease progression and emergence of
cognitive deficits. Herein we analyze functional and morphological changes in
isolated presynaptic terminals (synaptosomes) from frontal cortex of rats with
acute EAE. We show impairment in Ca2+-dependent L-glutamate
releaseconcomitant with alterations in the levels and phosphorylation state
ofsynapsin I and kinasesof the release machinery (Erk1/2 and CaMKII). These
changes rapidly reverse when the animals begin to recover from the clinical signs
of the disease. Our results indicate the release machinery is altered in the frontal
cortex of rats with EAE. Furthermore,electron microscopy studies revealed
differences in synaptic vesiclesdistribution and PSD size in EAE group. These are the
first evidences unraveling the molecular mechanism of presynaptic dysfunction in
frontal cortex during the course of EAE.