Apoptosis induction by glucuronoxylomannan of Cryptococcus neoformans.

CHIAPELLO L; AOKI MP; RUBINSTEIN HR; MASIH DT

TAYLOR & FRANCIS LTD

Año: 2003 vol. 41 p. 347 - 347

e studied the ability of glucuronoxylomannan (GXM), the major constituent ofCryptococcus neoformanscapsular polysaccharide, to induce apoptosis in lymphocytes from normal rats. Spleen mononuclear cells (Smc) from normal rats treatedwith GXM for 24 h exhibited, in comparison with controls, an increasedhypodiploidy in the DNA profile after staining with propidium iodide, as well asincreased ladder-type DNA fragmentation in agarose gel electrophoresis and ahigh number of positive cells in the terminal deoxynucleotidyl transferase biotindUTP nick end labeling (TUNEL) reaction. Furthermore, increased hypodiploidyin the DNA profile was also observed in Smc expressing T-cell receptor (TCR/).We also studied the induction of apoptosis in lungs and spleens from rats in theimmunosuppressor period of disseminated cryptococcosis. TUNEL labeling oflungs and spleens from rats obtained 14 days after infection with C. neoformansshowed a large