TLR2, TLR4 and TLR9 are differentially modulated in liver lethally injured from BALB/c and C57BL/6 mice during Trypanosoma cruzi acute infection

CARRERA SILVA A; CANO RC; GUIÑAZÚ N; AOKI MP; PELLEGRINI A; GEA S

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Londres; Año: 2008 vol. 45 p. 3580 - 3580

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt">Toll-like receptor (TLR) family is crucial for microbial elimination and homeostasis, and has an important immunoregulatory role. In this study, we comparatively analyze innate immune response and tisular injury elicited in BALB/c and C57BL/6 (B6) mice during acute Trypanosoma cruzi infection. The liver was the most affected tissue with numerous cellular infiltrates, apoptotic cells and necrotic areas. The apoptotic rate, evaluated by Hoescht stain, was highest in liver of B6. Infection increased transaminase activities in both mouse strains, although they were highest in B6. BALB/c showed six-fold higher parasitemias than B6 but the latter presented higher mortality (80%) than BALB/c (40%). To gain insight into the molecular basis, we investigated the TLRs commitment in liver. We found that, TLR2 and TLR4 were upregulatd in BALB/c while they were down-regulated in B6. However, TLR9 showe