AOKI MARIA DEL PILAR
Congresos y reuniones científicas
Título:
ROLE OF GALECTIN-8 IN THE OUTCOME OF TRYPANOSOMA CRUZI-INDUCED MYOCARDITIS
Autor/es:
BERTELLI, A; SANMARCO, LM; PASCUALE, C; POSTAN, M; AOKI, MP; LEGUIZAMÓN, S
Reunión:
Congreso; Reunión conjunta de Sociedades de Biociencias; 2017
Resumen:
Galectins are a family of lectins that contain characteristic aminoacid sequences and have affinity for B-galactosides. Galectin-8
(Gal-8), which belongs to the tandem repeat group, it is widely distributed in tissues and secreted to the extracellular media, acting in
an autocrine/paracrine manner. It participates in cellular adhesion,
migration, and apoptosis, mainly in cancer and autoimmune disorders. Different authors have also proposed Gal-8 to be involved
in pro-inflammatory or anti-inflammatory events. Chagasic cardiomyopathy is a chronic disease, whose development is based on
the induction of a strong inflammation by Trypanosoma cruzipersistence in cardiac tissue. The aim of this work was to analyze the
role of Gal-8 in an inflammatory context induced by T. cruzi.Gal-8
knock out (KO) and C57/BL6 (WT) mice were infected with T. cruzi
strain Ac and sacrificed at 4 months post infection for histopathology
and phenotyping analysis. The inflammation score in cardiac tissue
of KO mice was significantly higher compared with WT mice. No
difference was found in fibrosis induction. The cellular population
present in infected heart tissues, was characterized by flow-cytometry analysis. There were no differences in the levels of (CD-11b+Ly6C
highLy6G-) and (CD11b+Ly6C
low
Ly6G-) monocytes, dendritic cells (CD11c+F4/80-CD11b+) ;( CD11c+F4/80-CD11b-) and
T lymphocytes (CD3+CD4+ and CD3+CD8+) between KO and WT
infected hearts. However, inflammatory cells isolated from KO infected mice showed higher frequency of neutrophils (P=0.0097) and
macrophages (P=0.0021) compared to WT mice, with concomitant
increase of MCP-1 mRNA expression levels (P=0.0023). In agreement, increased levels of neutrophils (p=0.033) were observed in
spleens from KO infected mice compared to those of WT mice.
The reported participation of Gal-8 in preaperesis induction on leukocytes is in agreement with our findings.
Altogether, our results suggest a Gal-8 anti-inflammatory role in T.
cruziinfection
(Gal-8), which belongs to the tandem repeat group, it is widely distributed in tissues and secreted to the extracellular media, acting in
an autocrine/paracrine manner. It participates in cellular adhesion,
migration, and apoptosis, mainly in cancer and autoimmune disorders. Different authors have also proposed Gal-8 to be involved
in pro-inflammatory or anti-inflammatory events. Chagasic cardiomyopathy is a chronic disease, whose development is based on
the induction of a strong inflammation by Trypanosoma cruzipersistence in cardiac tissue. The aim of this work was to analyze the
role of Gal-8 in an inflammatory context induced by T. cruzi.Gal-8
knock out (KO) and C57/BL6 (WT) mice were infected with T. cruzi
strain Ac and sacrificed at 4 months post infection for histopathology
and phenotyping analysis. The inflammation score in cardiac tissue
of KO mice was significantly higher compared with WT mice. No
difference was found in fibrosis induction. The cellular population
present in infected heart tissues, was characterized by flow-cytometry analysis. There were no differences in the levels of (CD-11b+Ly6C
highLy6G-) and (CD11b+Ly6C
low
Ly6G-) monocytes, dendritic cells (CD11c+F4/80-CD11b+) ;( CD11c+F4/80-CD11b-) and
T lymphocytes (CD3+CD4+ and CD3+CD8+) between KO and WT
infected hearts. However, inflammatory cells isolated from KO infected mice showed higher frequency of neutrophils (P=0.0097) and
macrophages (P=0.0021) compared to WT mice, with concomitant
increase of MCP-1 mRNA expression levels (P=0.0023). In agreement, increased levels of neutrophils (p=0.033) were observed in
spleens from KO infected mice compared to those of WT mice.
The reported participation of Gal-8 in preaperesis induction on leukocytes is in agreement with our findings.
Altogether, our results suggest a Gal-8 anti-inflammatory role in T.
cruziinfection
