Hematopoiesis and thymic apoptosis are not affected by the loss of Cdk2

BERTHET C, RODRIGUEZ GALAN MC, HODGE DL, GOOYA J, PASCAL V, YOUNG HA, KELLER J, BOSSELUT R, KALDIS P.

AMER SOC MICROBIOLOGY

Año: 2007 vol. 27 p. 5079 - 5079

b> Cell cycle regulation is essential for proper homeostasis of hematopoietic cells. Cdk2 is a major regulator of S phase entry, is activated by mitogenic cytokines, and has been suggested to be involved in antigen-induced apoptosis of T lymphocytes. The role of Cdk2 in hematopoietic cells and apoptosis in vivo has not yet been addressed. To determine whether Cdk2 plays a role in these cells, we performed multiple analyses of bone marrow cells, thymocytes, and splenocytes from Cdk2 knockout mice. We found that Cdk2 is not required in vivo to induce apoptosis in lymphocytes, a result that differs from previous pharmacological in vitro studies. Furthermore, thymocyte maturation was not affected by the lack of Cdk2. We then analyzed the hematopoietic stem ce