Safety levels of systemic IL-12 induced by cDNA expression as cancer therapeutic

CONSTANZA SAVID-FRONTERA; VIANO, MARIA ESTEFANIA; BAEZ, NATALIA S.; DELLA REYNOLDS; MARIANA MATELLON; YOUNG, HOWARD A.; MARIA CECILIA RODRIGUEZ GALAN

IMMUNOTHERAPY

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2021

1750-743X

ims: The aim of this work is to utilize a gene expression procedure to safety express systemic IL-12 and evaluate its effects in mouse tumor models. Materials & Methods: SLO and tumors from EL4 and B16 tumor bearing mice were analyzed by supervised and unsupervised methods. Results: IL-12 cDNA induced systemic IL-12 protein levels lower than the tolerated dose in patients. Control of tumor growth is observed in s.c. B16 and EL-4 tumors. Systemic IL-12 expression induced a higher frequency of both total tumor-infiltrated CD45+ cells and proliferative IFN+CD8+T cells along with a lower frequency of CD4+Foxp3+ and CD11b+Gr-1+ cells. Conclusions: This approach characterizes the systemic effects of IL-12 helping to improved treatment of metastases or solid tumors.