Adsorption and electroxidation of DNA at glassy carbon electrodes modified with carbon nanotubes dispersed in glucose oxidase.

FABIANA A. GUTIÉRREZ; MARÍA D. RUBIANES; GUSTAVO A. RIVAS.

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 25 p. 1135 - 1135

e are proposing for the first time the successful  immobilization of DNA at glassy carbon electrodes (GCE) modified with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorption or by layer by layer self assembling using polydiallyldimethylamine (PDDA). The presence of GOx allows an efficient dispersion of CNT and gives to the platform a most favorable environment that promotes the adsorption and further electrooxidation of DNA. The PDDA incorporated in the self assembled architecture largely facilitates the adsorption of dsDNA and the adsorbed layer can be successfully used for evaluating the interaction of DNA with methylene blue by electrochemical techniques.