Vacuolar Protein Sorting Receptor in Giardia lamblia

MARIA ROMINA RIVERO; SILVANA MIRAS; CONSTANZA FELIZIANI; NAHUEL ZAMPONI; RODRIGO QUIROGA; STANLEY HAYES; ANDREA RÓPOLO; MARÍA C TOUZ

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2012 vol. 7 p. 43712 - 43712

p align="LEFT">In Giardia, lysosome-like peripheral vacuoles (PVs) need to specifically coordinate their endosomal and lysosomal functions to be able to successfully perform endocytosis, protein degradation and protein delivery, but how cargo, ligands and molecular components generate specific routes to the PVs remains poorly understood. Recently, we found that delivering membrane Cathepsin C and the soluble acid phosphatase (AcPh) to the PVs is adaptin (AP1)-dependent. However, the receptor that links AcPh and AP1 was never described. We have studied protein-binding to AcPh by using H6-tagged AcPh,