Excretory-Secretory Products from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism.

M C SERRADELL, L GUASCONI, L CERVI, LS CHIAPELLO, DT MASIH.

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Año: 2007 vol. 117 p. 197 - 197

0165-2427

p class="MsoNormal" style="MARGIN: 0cm 0cm 10pt">Eosinophils (Eo) are known to be important effector cells in the host defense against helminth parasites. Excretory-secretory products (ESP) released by helminths have shown wide immunomodulatory properties, such as the induction of cellular apoptosis. We investigated the ability of ESP from Fasciola hepatica to induce Eo apoptosis. In this work, we observed that ESP induced an early apoptosis of rat peritoneal eosinophils and that this phenomenon was time- and concentration-dependent. Furthermore, we demonstrated that activation of protein tyrosine kinases (TyrK) and caspases were necessary to mediate the Eo apoptosis induced by the ESP, and that carbohydrate components present in these antigens were involved in this effect.We have described for the first time the ability of ESP from F. hepatica to modify the viability of Eo by apoptosis induction. Besides that, we h