Immunomodulatory Effect of Fasciola hepatica Excretory?Secretory Products on Macrophages

GUASCONI L; SERRADELL M; MASIH DT; CHIAPELLO L S; MARTÍN CANCELA; GABRIELA MAGGIOLI

Methods in Molecular Biology. Fasciola hepatica: Methods and Protocols

Humana, New York, NY

Lugar: New York; Año: 2020; p. 117 - 131

The liver fluke, Fasciola hepatica, infects a wide range of mammals including man, leading to chronic disease. Alike other helminthes, F. hepatica migrates and survives in the host tissues after penetrate intestinal wall into the peritoneal cavity and then migrate through the liver before finally inhabiting the bile ducts. To avoid the anti-heminthic immune response during migration, F. hepatica releases excretory-secretory products (FhESP) that dampens pro-inflamatory functions of innate immune cells, such as alternative macrophage activation or programmed cell death induction. Here, we describe techniques to study macrophage activation and apoptotic cell death triggered by purified FhESP from adult Fasciola hepatica fluke.