Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality.

GUYÓN, NOELIA; BISTONI, MARÍA DE LOS ANGELES; WUNDERLIN, DANIEL ALBERTO; AMÉ, MARÍA VALERIA

Journal of the Brazilian Society of Ecotoxicology

J. Braz. Soc. Ecotoxicol

Año: 2012 vol. 7 p. 97 - 97

1809-8401

p class="MsoNormal" style="line-height: 200%; margin: 0cm 0cm 0pt;">Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, wat