Effects of Microcystin-LR on the Expression of P-glycoprotein in Jenynsia multidentata.

AMÉ, MARÍA VALERIA; BARONI, MARÍA VERÓNICA; GALANTI, LUCAS NICOLÁS; BOCCO, JOSE LUIS; WUNDERLIN, DANIEL ALBERTO

Elsevier

Lugar: Amsterdam; Año: 2009 vol. 74 p. 1179 - 1179

font size="1" face="AdvGulliv-R"> The multixenobiotic resistance phenomenon (MXR) related to the P-glycoprotein multidrug transporter protein (P-gp) has been identified and characterized in several aquatic organisms. In the present work, we prove the presence of a P-gp in liver, gills and brain of Jenynsia multidentata by Western Blot and RT?PCR. A 170 kDa protein has been found in liver and gills while in brain a 80 kDa protein has been detected. The partial nucleotide sequence obtained in this autochthonous fish showed high similarity ranging from 83% to 92% with other fishes. In addition, P-gp expression in this fish was evaluated after time and dose-dependent exposures to the cyanotoxin microcystin?LR. Individuals were exposed to MC?LR at concentrations of 2, 5 and 10