Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors.

MULLER, VIRGINIA; BONACCI, GUSTAVO; BATTHYANY, CARLOS; AMÉ, MARÍA VALERIA; CARRARI, FERNANDO; GIECO, JORGE; ASIS, RAMÓN

PHYTOPATHOLOGY

AMER PHYTOPATHOLOGICAL SOC

Año: 2017 vol. 107 p. 474 - 474

0031-949X

ignificant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI337394 and the susceptible Forman cultivar during A.parasiticus colonization. With chromatographic methods and 2D electrophoresis-massspectrometry we have isolated and identified four variants of Bowman-Birk trypsin inhibitor (BBTI) and a novel Kunitz-type protease inhibitor (KPI) produced in response to A. parasiticus colonization. KPI was detected only in the resistant cultivar while BBTI was produced in the resistant cultivar in a higher concentration than susceptible cultivar and with different isoforms. The kinetic expression of KPI and BBTI genes along with trypsin inhibitory activity was analyzed in both cultivars durin