Selection of reference genes for RT-qPCR analysis in the biomonitor macrophyte Bidens laevis L.

LUKASZEWICZ, GERMÁN; AMÉ, MARÍA VALERIA; MENONE, MIRTA L.

Physiology and Molecular Biology of Plants

Springer India

Lugar: New Delhi ; Año: 2018 p. 1 - 1

he RT-qPCR has been the method used to analyze gene expression in plants but its benefits have not been completely exploited in the field of plants ecotoxicology when used as molecular biomarkers. The correct use of RT-qPCR demands to establish a certain number of Reference Genes (RG) which are expected to be invariable in their expression although it does not always happen. The main goals of this work were to: -analyze the stability of six potential RG, -establish the optimum number of RG, -select the most suitable RG to be applied in Bidens laevis under different test conditions and tissues and -confirm its convenience by normalizing the expression of one Gene of Interest (GOI) under three different challenges. When all data were pooled together, the geNorm algorithm pointed out beta- actin (BACT) and beta- tubulin (TUB) as the optimal RG pair while NormFinder algorithm selected Nicotinamide Adenine Dinucleotide Dehydrogenase (NADHD) and Histone 3 (H3) as having the most invariable