Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. exposed to a commercial formula of endosulfan

PEREZ, DÉBORA; LUKASZEWICZ, GERMÁN; MENONE, MIRTA; AMÉ, MARÍA VALERIA; CAMADRO, ELSA

JOHN WILEY & SONS INC

Lugar: New York; Año: 2014 vol. 29 p. 1063 - 1063

revious studies in the wetland macrophyte Bidens laevis L have demonstrated that theinsecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase?telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of this study were to use cytogenetic biomarkers? CAAT and abnormal metaphase?and defense biomarkers such as the activity of the antioxidant enzymes?guaiacol peroxidases (POD), glutathione reductase, and microsomal and cytosolic (m- and c-) glutathione-S-transferase (GST)?to evaluate in B. laevis effects caused by a commercial formulation of endosulfan. The frequency of CAAT was increased at 5, 10, 50, and 100 ug/L endosulfan with respect to the negative controls by 3.1, 2.5, 2.5, and 3.2-fold, respectively while the frequency of abnor